| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Lim Laurie, Maia K (9)
|
MRAGUA |
40.87 |
39.74 |
-1.13 |
| 2 |
Torres, Sofia R (9)
|
MRWEST |
41.53 |
40.54 |
-0.99 |
| 3 |
Arciniegas, Alessandra (9)
|
MRCOND |
38.76 |
40.68 |
1.92 |
| 4 |
Wong, Cailin (9)
|
MRNYCC |
41.84 |
41.02 |
-0.82 |
| 5 |
Plawiak, Emilia A (9)
|
MRYMID |
41.80 |
41.07 |
-0.73 |
| 6 |
Lee, Yuna (9)
|
MRLIAC |
42.88 |
41.65 |
-1.23 |
| 7 |
Messinger-Nanni, Julia (9)
|
MRAGUA |
42.48 |
41.85 |
-0.63 |
| 8 |
Johnson, Evangeline N (9)
|
MRAGUA |
43.07 |
42.55 |
-0.52 |
| 9 |
Jorsling, Jisella C (9)
|
MRBAD |
42.40 |
42.84 |
0.44 |
| 10 |
Briceno, Isabella (9)
|
MRNYSA |
43.17 |
43.21 |
0.04 |
| 11 |
McDonald, Keira M (9)
|
MRBAD |
43.07 |
43.61 |
0.54 |
| 12 |
Curinnaia, Elena (8)
|
MRMPNY |
42.84 |
43.84 |
1.00 |
| 13 |
Li, Summer A (9)
|
MRAPEX |
42.92 |
44.54 |
1.62 |
|
Wang, Louisa M (9)
|
MRWEST |
43.05 |
NS |
- |